Cell Fractionation Golgi bottom fraction

Found In:

15817825

Description

Title: Cell Fractionation Golgi bottom fraction
Creator: Bergeron, John
Palade, George E
Siekevitz, Philip
Contributor: Jamieson, James D
Published / Created: 11/11/1970
Publication Place: New Haven, CT
Publisher: Yale University School of Medicine
Description: 3.25 in. x 4 in. Lantern Slides
Original Magnification: x13,750
The authors took advantage of the fact that rats gavage-fed a large bolus of ethanol (single malt I hope) were observed to have their Golgi cisternae loaded with LDL particles. Since LDL particles are lighter than most other intracellular organelles, following homogenization of ethanol-fed rats, the Golgi fractions "floated" to the top of denser sucrose gradients during centrifugation. This allowed investigators to analyze the membrane properties of "pure" Golgi fractions and set the stage for development of fusion assays that led to an understanding of factors regulating the specificity of membrane fusion - the SNARE hypothesis.
Extent: 3.25 in. x 4 in.

Access: Public
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Yale Community Only
Citation: See Ehrenreich, J.H., J.J.M. Bergeron, P. Siekevitz and G.E. Palade. 1973. Golgi fractions prepared from rat liver homogenates: I. Isolation procedure and morphological characterization. J. Cell. Biol. 59:45-72.