Guinea pig pancreatic slice in vitro EMAR 3 min. pulse

Found In:

15817882

Description

Title: Guinea pig pancreatic slice in vitro EMAR 3 min. pulse
Creator: Jamieson, James D
Contributor: Jamieson, James D
Publication Place: New Haven, CT
Publisher: Yale University School of Medicine
Description: 3.25 in. x 4 in. Lantern Slides
Original Magnification: x17,000
Pulse chase experiments, carried out by Jamieson and Palade, defined the kinetics and pathway of intracellular transport of newly synthesized proteins in guinea pig pancreatic slices in vitro. The 3 min pulse consisted of preincubation in the cold in a leucine-free medium containing tritiated leucine, followed by warming up to 37 for 3 min. Chase incubations followed by incubation in medium containing an excess of "cold" leucine, fixation at indicated times, and processing for EM autoradiography. Historic details in the Nobel Lecture 1974 by Dr. Palade.
Extent: 3.25 in. x 4 in.

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