3.25 in. x 4 in. Lantern Slides, Original Magnification: x1,000, and The purpose of the technique was to isolate a population of viable single pancreatic acinar cells that were representative of the acinar cells from the entire gland. An additional benefit of a slurry of individual cells was rapidity and ease of washing and manipulation of the cell suspension. The technique employed mild collagenase digestion and trituration of the digested guinea pig pancreas. Functional and morphologic characterization is described in the paper.
3.25 in. x 4 in. Lantern Slides and Classic TEM. First description of the endoplasmic reticulum. Whole mount of unfixed, dried chick embryo fibroblast. The genius of their approach was to grow embryonic chick cells on a thin collodion film supported by a fine mesh copper grid. After the cells had grown and spread out thin processes, the preparation was air dried. They reasoned that the thin processes would allow electrons to penetrate in the electron microscope. The only microscope available to them was owned by the Interchemical Corp. and housed in the Empire State Building. Fullam was the technician. The electron microscope was a first generation RCA type B.
