3.25 in. x 4 in. Lantern Slides, One of Dr. Palade's long standing interests concerned the structure and function of capillaries. He and his many colleagues identified a variety of types of capillary endothelium (fenestrated, continuous) and determined the role of caveolae in transporting macromolecules across endothelial cells. Collaborators included: Nicolae and Maya Simeonescu and Romaine Bruns., and Original Magnification: x88,000
3.25 in. x 4 in. Lantern Slides, Clathrin coated pit caveola and transgential cut of coated vesicle in endothelial cell., and Original Magnification: x248,000
3.25 in. x 4 in. Lantern Slides, Discontinuous capillary; guinea pig pancreas; pericyte under the endothelium. Red blood cell in lumen., and Original Magnification: x17,000
3.25 in. x 4 in. Lantern Slides, Capillary lumen rat diaphragm 24 hr. post IV ferritin. Some ferritin in intracellular vesicles and extracellular space., and Original Magnification: x25,000
3.25 in. x 4 in. Lantern Slides, Capillary rat diaphragm 2h post IV ferritin. Ferritin is seen entering plasmalemmal vesicles and into the extracellular space., and Original Magnification: x62,000
3.25 in. x 4 in. Lantern Slides, Original Magnification: x13,750, and The authors took advantage of the fact that rats gavage-fed a large bolus of ethanol (single malt I hope) were observed to have their Golgi cisternae loaded with LDL particles. Since LDL particles are lighter than most other intracellular organelles, following homogenization of ethanol-fed rats, the Golgi fractions "floated" to the top of denser sucrose gradients during centrifugation. This allowed investigators to analyze the membrane properties of "pure" Golgi fractions and set the stage for development of fusion assays that led to an understanding of factors regulating the specificity of membrane fusion - the SNARE hypothesis.
